Publications

2024

Sakoguchi, A.†, Chamberlain, S.G.†, Mørch, A.M., Harrison, T.E., Dustin, M.L., Arase, H.*, Higgins, M.K.*, Iwanaga S.* (2024) RIFINs displayed on malaria-infected erythrocytes bind both KIR2DL1 and KIR2DS1. BioRXIV doi.org/10.1101/2024.04.30.591854 (†equal contribution; * corresponding)

Williams, B.G., King, L.D.W., Pulido, D., Quinkert, D., Lias, A.M., Silk, S.E., Ragotte, R.J., Davies, H., Barrett, J.R., McHugh, K., Rigby, C.A., Alanine, D.G.W., Baford, L., Shea, M.W., Cowley, L.A., Dabbs, R.A., Pattinson, D.J., Douglas, A.D., Lyth, O.R., Illingworth, J.J., Jin, J., Carnrot, C., Kotraiah, V., Christen, J.M., Noe, A.R., MacGill, R.S., King, C.R., Birkett, A.J., Soisson, L.A., Skinner, K., Miura, K., Long, C.A., Higgins, M.K., Draper, S.J. (2024) Development of an improved blood-stage malaria vaccine targeting the essential RH5-CyRPA-RIPR invasion complex. BioRXIV doi.org/10.1101/2024.02.08.579322

Harrison, T.E.*, Alam, N.*, Farrell, B.*, Quinkert, D., Lias, A.M., King, L.D., Draper, S.J., Campeotto, I. and Higgins, M.K. (2024) Rational structure-guided design of a blood stage malaria vaccine immunogen presenting a single epitope from PfRH5. https://doi.org/10.1101/2024.02.29.582763 (* contributed equally)

Colomb. F.,  Ogunkanbi, A.,  Jamwal, A., Dong, B.,  Maizels, R.M., M.Finney, C.A.,  Wasmuth, J.D.,  Higgins, M.K. and  McSorley, H.J. (2024) IL-33-binding HpARI family homologues with divergent effects in suppressing or enhancing Type 2 immune responses. Infect Immun. 31 e0039523

Farrell, B., Alam, N., Hart, M.N., Jamwal, A., Ragotte, R.J., Walters-Morgan, H., Draper, S.J., Knuepfer, E. and Higgins, M.K. (2024) Structure of the PfRCR complex which bridges the malaria parasite and erythrocyte during invasion. Nature 625 578-584

 

2023

Wang, L.T.*, Cooper, A.*, Farrell, B.*, Miura, K., Diouf, A., Muller-Sienerth, N., Crosnier, C., Purser, L., Maciuszek, M., Barret, J.R., McHugh, K., Tucker, C., Li, S., Doumbo, S., Doumtabe, D., Pyo, C.-P., Nielsen, C.M., Silk, S.E., Kayentao, K., Ongoiba, A., Nguyen, D.C., Lee, F.E., Minassian, A.M., Geraghty, D.E., Traore, B., Seder, R.A., Crompton, P.D., Wright, G., Draper, S.J., Long, C., Higgins, M.K. and Tan, J. (2023) Natural malaria infection elicits rare but potent neutralizing antibodies to the blood-stage antigen RH5. BioRXIV doi.org/10.1101/2023.10.04.560669 (* contributed equally)

Jamwal, A., Colomb, F., McSorley, H.J.* and Higgins, M.K.* (2023) Structural basis for IL-33 recognition and its antagonism by the helminth effector protein HpARI. BioRXIVdoi.org/10.1101/2023.08.10.552813

Moskovitz, R.†, Pholcharee, T.†, DonVito, S.M., Guloglu, B., Lowe, E., Mohring, F., Moon, R.W.  and Higgins, M.K. (2023) Structural basis for DARC binding in reticulocyte invasion by Plasmodium vivax. Nature Communications 14 3637

Cook, A.D., Carrington, M.* and Higgins, M.K.* (2023) Trypanosomes and complement: more than one way to die? Trends in Parasitology 39 1014

Cook, A.D., Carrington, M.* and Higgins, M.K.* (2023) Molecular mechanism of complement inhibition by the trypanosome receptor ISG65. eLife doi.org/10/7554/eLife.88960.1 

Jamwal, A., Constantin, C., Henrich, S., Bildl, W., Faklerrr, B., Draper, S.J., Schulte, U., Higgins, M.K. (2023) Erythrocyte invasion-neutralising antibodies prevent Plasmodium falciparum RH5 from binding to basigin-containing membrane protein complexes. eLife 12 e83681

 

2022

Ko, K.T., Lennartz, F., Mekhaiel, D., Guloglu, B., Marini, A., Deuker, D.J., Long, C.A., Jore, M.M., Miura, M., Biswas, S. and Higgins, M.K. (2022) Structure of the malaria vaccine candidate Pfs48/45 and its recognition by transmission blocking antibodies. Nature Communications 13 5603

Macleod, O.J.+, Cook, A.D.+, Webb, H., Crow, M., Burns, R., Redpath, M., Seisenberger, S., Trevor, C.E., Peacock, L., Schwede, A., Kimblin, N., Francisco, A.F., Pepperl., Rust, S., Voorheis, P., Gibson, W., Taylor, M.C., Higgins, M.K.* and Carrington, M.* (2022) Invariant surface glycoprotein 65 of Trypanosoma brucei is a complement C3 receptor. Nature Communications 13 5085 (+ equal contribution, * joint corresponding)

Giorgalli, M., Cunningham, D.A., Broncel, M., Sait, A., Harrison, T.E., Hosking, C., Vandomme, A., Amis, S.I., Antonello, A., Sullivan, L., Uwadiae, F., Torella, L., Higgins, M.K. and Langhorne, J. (2022) Differential Trafficking and Expression of PIR Proteins in Acute and Chronic Plasmodium Infections. Front Cell Infect Microbiol. 12 877253

Ragotte, R.J., Pulido, D., Lias, A.M., Quinkert, D., Alanine, D.G.W., Jamwal, A., Davies, H., Nacer, A., Lowe,E.D., Grime, G.W., Illingworth, J.J., Donat, R.F, Garman, E.F., Bowyer, P.W., Higgins, M.K.*, Draper, S.J.* (2022) Heterotypic interactions drive antibody synergy against a malaria vaccine candidate. Nature Communications 13 933 (* joint corresponding)

 

2021

Cook, A.D. and Higgins, M.K. (2021) High-throughput hit-squad tackles trypanosomes. Trends in Parasitology 37 772-4

Higgins, M.K. (2021) Can we AlphaFold our way out of the next pandemic? J. Mol. Biol. 8 167093

Higgins, M.K. (2021) Why it might be bad for brain cells to eat malaria parasites. Journal of Experimental Medicine 218 e20202664

Barber, N.M., Lau, C.Y.K., Turner, L., Watson, G., Thrane, S., Lusingu, J.P.A., Lavstsen, T.* and Higgins, M.K.* (2021) Structure-guided design of a synthetic mimic of an EPCR-binding PfEMP1 protein. mSphere 6 e01081-20 (* joint corresponding)

 

2020

Harrison, T.E., Reid, A.J., Cunningham, D., Langhorne, D. and Higgins, M.K. (2020) Structure of the Plasmodium-interspersed repeat proteins of the malaria parasite. Proc Natl Acad Sci U S A 117 32098-32104

Higgins, M.K. (2020) Staging an antibody contest to fight malaria. Immunity 53 697-699

Harrison, T.E., Mørch, A.M., Felce, J.H., Sakoguchi, A., Reid, A.J., Arase, H., Dustin, M.L. and Higgins, M.K. (2020) Structural basis for RIFIN-mediated activation of LILRB1 in malaria. Nature 587 309-312

Gérard, S.F., Hall, B.S., Zaki, A.M., Corfield, K.A., Mayerhofer, P.U., Costa, C., Whelligan, D.K., Biggin, P.C., Simmonds, R.E.* and Higgins, M.K.* (2020) Structure of the inhibited state of the Sec translocon. Molecular Cell 79 406-415 (* joint corresponding)

Campeotto, I., Galaway, F., Mehmood, S., Barfod, L.K., Quinkert, D., Kotraiah, V., Phares, T.W., Wright, K.E., Snijders, A.P., Draper, S.J., Higgins, M.K.* and Wright, G.J.* (2020) Structure of the cysteine-rich domain of Plasmodium falciparum P113 identifies the location of the RH5 binding site. mBio 11 e01566-20 (* joint corresponding)

Alam, N and Higgins, M.K. (2020) A spike with which to beat COVID-19. Nature Reviews Microbiology 18 414

Vacca, F., Chauché, C., Jamwal, A., Hinchy, E.C., Heieis, G., Webster, H., Ogunkanbi, A., Sekne, Z., Gregory, W.F., Wear, M., Perona-Wright, G., Higgins, M.K., Nys, J.A., Cohen, E.S. and McSorley H.J. (2020) A helminth-derived suppressor of ST2 blocks allergic responses. eLife 9 e54017

Ragotte, R.J., Higgins, M.K. and Draper, S.J. (2020) The RH5-CyRPA-Ripr complex as a malaria vaccine target. Trends in Parasitology 36 545-559

Macleod, O.J.S., Bart, J., MacGregor, P., Peacock, L., Savill, N.J., Hester, S., Ravel, S., Sunter, J.D., Trevor, C., Rust, S., Vaughan, T.J., Minter, R., Mohammed, S., Gibson, W., Taylor, M.C., Higgins, M.K.* and Carrington, M.* (2020) A receptor for the complement regulator factor H increases transmission of trypanosomes to tsetse flies. Nat Commun 11, 1326 (* joint corresponding)

 

2019

Trevor, C., Gonzalez-Munoz, A.L., MacLeod, O.J.S., Woodcock, P.G., Rust, S., Vaughan, T.J., Garman, E.F., Minter, R., Carrington, M.* and Higgins, M.K.* (2019) Structure of the trypanosome transferrin receptor reveals mechanisms of ligand recognition and immune evasion. Nature Microbiology 10.1038/s41564-019-0589-0 (* joint corresponding)

Lennartz, F., Smith, C., Craig, A.G. and Higgins, M.K. (2019) Structural insights into diverse modes of ICAM-1 binding by Plasmodium falciparum-infected erythrocytes. Proc. Natl. Acad. Sci. USA 116 20124-20134

Warszawski, S., Dekel, E., Campeotto, I., Marshall, J.M., Wright, K.E., Lyth, O., Knop, O., Regev-Rudzki, N., Higgins, M.K., Draper, S.J., Baum, J. and Fleishman, S.J. (2019) Design of a basigin-mimicking inhibitor targeting the malaria invasion protein RH5. Proteins doi: 10.1002/prot.25786

Illingworth, J.J., Alanine, D.G., Brown, R., Marshall, J.M., Bartlett, H.E., Silk, S.E., Labbé, G.M., Quinkert, D., Cho, J.S., Wendler, J.P., Pattinson, D.J., Barfod, L., Douglas, A.D., Shea, M.W., Wright, K.E., de Cassan, S.C., Higgins, M.K. and Draper, S.J. (2019) Functional Comparison of Blood-Stage Plasmodium falciparum Malaria Vaccine Candidate Antigens. Front Immunol. 10 1254

Alanine, D.G.W, Quinkert, D., Kumarasingha, R., Mehmood, S., Donnellan, F.R., Minkah, N.K., Dadonaite, B., Diouf, A., Galaway, F., Silk, S.E., Jamwal, A., Marshall, J.M., Miura, K., Foquet, L., Elias, S.C., Labbé, G.M., Douglas, A.D., Jin, J., Payne, R.O., Illingworth, J., Pattinson, D.J., Pulido-Gomez, D., Williams, B.G., de Jongh, W.A., Wright, G.J., Kappe, S.H.I., Robinson, C.V., Long, C.A., Crabb, B.S., Gilson, P.R., Higgins, M.K.* and Draper, S.J.*. (2019) Human antibodies that slow erythrocyte invasion potentiate malaria-neutralizing antibodies. Cell 178 216-228 (* joint corresponding)

Knuepfer, E., Wright, K.E., Kumar Prajapati, S., Rawlinson, T.A., Mohring, F., Koch, M., Lyth, O.R., Howell,S.A., Villasis, E., Snijders, A.P., Moon, R.W., Draper, S.J., Rosanas-Urgell, A., Higgins, M.K., Baum, J., Holder, A.A. (2019) Divergent roles for the RH5 complex components, CyRPA and RIPR in human-infective malaria parasites. PLoS Pathogens 15 e1007809

Rawlinson, T.A., Barber, N.M., Mohring, F., Cho, J.S., Kosaisavee, V., Gérard, S.F., Alanine, D.G.W., Labbé, G.M., Elias. S.C., Silk, S.E., Quinkert, D., Jin, J., Marshall, J.M., Payne, R.O., Minassian, A.M., Russell, B., Rénia, K., Nosten. F.H., Moon, R.W., Higgins, M.K.* and Draper, S.J.* (2019) Structural basis for inhibition of Plasmodium vivax invasion by a broadly neutralising vaccine-induced human antibody. Nature Microbiology doi: 10.1038/s41564-019-0462-1 (*joint corresponding)

MacGregor, P., Gonzalez-Munoz, A.L., Jobe, F., Taylor, M., Rust, S., Sandercock, A.M., Macleod, O.J.S., Van Bocxlaer, K., Francisco, A.F., D’Hooge, F., Tiberghien, A., Barry, C.S., Howard, P., Higgins, M.K., Vaughan, T.J., Minter, R. and Carrington, M. (2019) A single dose of targeted antibody-toxin conjugate cures a mouse model of African trypanosomiasis. PLoS Neglected Tropical Disease 13 e0007373

 

 

2018

Lennartz, F., Brod, F., Dabbs, R., Miura, K., Mekhaiel, D., Marini, A., Jore, M.M., Søgaard, M.M., Jørgensen, T., de Jongh, W.A., Sauerwein, R.W., Long, C.A., Biswas, S. and Higgins, M.K. (2018) Structural basis for recognition of the malaria vaccine candidate Pfs48/45 by a transmission blocking antibody. Nature Communications 9 3822

Carrington, M and Higgins, M.K. (2018) O-h what a surprise. Nature Microbiology 3 856-857

Draper, S.J., Sack, B.K., King, C.R., Nielsen, C.M., Rayber, J.C., Higgins, M.K., Long, C.A. and Seder, R.A. (2018) Malaria vaccines: recent advances and new horizons. Cell Host and Microbe 24 43-56

Draper, S.J. and Higgins, M.K. (2018) A new site of attack for a malaria vaccine. Nature Medicine 24 382-3

Carrington, E., Otto, T.D., Szestak, T., Lennartz, F., Higgins, M.K., Newbold, C.I. and Craig, A.G. (2018) In silico guided reconstruction and analysis of ICAM-1-binding var genes from Plasmodium falciparum. Sci Rep. 8 3282

Zoll, S., Lane-Serff, H., Mehmood, S., Robinson, C.V., Carrington, M. and Higgins, M.K. (2018) The structure of the serum resistance associated protein of Trypanosoma brucei rhodesiense and its implications for human infectivity. Nature Microbiology 3 295-301

 

 

2017

Lennartz, F., Lavstsen, T. and Higgins, M.K. (2017) Towards an anti-disease malaria vaccine. Emerging topics in Life Sciences 1 539-45

Higgins, M.K. and Lea, S.M. (2017) On the state of crystallography at the dawn of the electron microscopy revolution. Current Opinions in Structural Biology 46 95-101

Hsieh, F.-L. and Higgins M.K. (2017) The structure of a LAIR1-containing human antibody reveals a novel mechanism of antigen recognition. eLife 6 e27311

Lennartz, F., Adams, Y., Bengtsson, A., Olsen, R.W., Turner, L., Ndam, N.T., Ecklu-Mensah, G., Moussiliou, A., Ofori, M.F., Gamain, B., Lusingu, J.P., Petersen, J.E.V, Wang, C.W., Nunes-Silva, S., Jespersen, J.S., Lau, C.K.W., Theander, T.G., Lavstsen, T., Hviid, L., Higgins, M.K.* and Jensen,A.T.R * (2017) Structure-guided identification of a family of dual receptor binding PfEMP1 that are associated with cerebral malaria. Cell Host and Microbe 21 403-414  (* joint corresponding).

Jin, J., Hjerrild, K.A., Silk, S.E., Brown, R.E., Labbé, G.M., Marshall, J.M., Wright, K.E., Bezemer, S., Clemmensen, S.B., Biswas, S., Li, Y., El-Turabi, A., Douglas, A.D., Hermans, P., Detmers, F.J., de Jongh, W.A., Higgins, M.K., Ashfield, R. and Draper, S.J. (2017) Accelerating the clinical development of protein-based vaccines for malaria by efficient purification using a four amino acid C-terminal 'C-tag'. Int J Parasitol. S0020-7519 30018-8

Campeotto, I., Goldenzweig, A., Davey, J., Barfod, L., Marshall, J.M., Silk, S.E., Wright, K.E., Draper, S.J., Higgins, M.K.* and Fleishman, S.J.* (2017) One-step design of a stable variant of the malaria invasion protein RH5 for use as a vaccine immunogen. Proc Natl Acad Sci U S A. 114 998-1002 (* joint corresponding).

Higgins, M.K.,Lane-Serff, H., MacGregor, P. and Carrington, M. (2017) A receptor’s tale: an eon in the life of a trypanosome receptor. PLoS Pathogen 13 e1006055

 

 

2016

Hsieh, F., Turner, L., Bolla, J.L., Robinson, C.V., Lavstsen, T. and Higgins, M.K. (2016) The structural basis for CD36 binding by the malaria parasite. Nature Comm 7 12837

Hjerrild, K.A., Jin, J., Wright, K.E., Brown, R.E., Marshall, J.M., Labbé, G.M., Silk, S.E., Cherry, C.J., Clemmensen, S.B., Jorgensen, T., Illingworth, J.J., Alanine, D.G.W., Milne, K.H., Ashfield, R., de Jongh, W.A., Douglas, A.D., Higgins, M.K.*, and Draper, S.J.* (2016) Production of full-length soluble Plasmodium falciparum RH5 protein vaccine using a Drosophila melanogaster Schneider 2 stable cell line system. Scientific Reports 6 30357 (* joint corresponding).

Lane-Serff, H., MacGregor, P., Peacock, L., Macleod, O.J., Kay, C., Gibson, W., Higgins, M.K.* and Carrington, M.* (2016) Evolutionary diversification of the trypanosome haptoglobin-haemoglobin receptor from an ancestral haemoglobin receptor. eLife 5 e13044 (* joint corresponding).

 

 

2015

Chakrabarti, K.S., Agafonov, R.V., Pontiggia, F., Otten, R., Higgins, M.K., Schertler, G.F.X., Oprian, D.D. and Kern, D. (2015) Conformational Selection in a Protein-Protein Interaction revealed by Dynamic Pathway Analysis. Cell Reports 14 32-42

Lennartz, F., Bengtsson, A., Olsen, R.W., Joergensen, L., Brown, A., Remy, L., Man, P., Forest, E., Barfod, L.K., Adams, Y., Higgins, M.K.* and Jensen A.T.* (2015) Mapping the binding site of a cross-reactive Plasmodium falciparum PfEMP1 mAb inhibitory of ICAM-1 binding. J. Immunol. 195 3273-3283 (* joint corresponding).

Lau, C.K.Y., Turner, L., Jespersen, J.S., Lowe, E.D., Petersen, B., Wang, C.W., Petersen, J.E.V., Lusingu, J., Theander, T.G., Lavstsen, T. and Higgins, M.K. (2015) Structural conservation despite huge sequence diversity allows EPCR binding by the PfEMP1 family implicated in severe childhood malaria. Cell Host and Microbe 17 118-129

 

 

2014

Lane-Serff, H., McGregor, P., Lowe, E.D., Carrington, M. and Higgins, M.K. (2014) Structural basis for ligand and innate immunity factor uptake by the trypanosome haptoglobin-haemoglobin receptor. eLife 3 e05553

Wright, K.E., Hjerrild, K.A., Bartlett, J., Douglas, A.D., Jin, J., Brown, R.E., Illingworth, J.J., Ashfield R., Clemmensen, S.B., de Jongh, W.A., Draper, S.J. and Higgins, M.K. (2014) Structure of malaria invasion protein RH5 with erythrocyte basigin and blocking antibodies. Nature 515 427-30

Thomson, R., Genovese, G., Canon, C., Kovacsics, D., Higgins, M.K., Carrington, M., Winkler, C.A., Kopp, J., Rotimi, C., Adeyemo, A., Doumatey, A., Ayodo, G., Alper, S.L., Pollak, M.R., Friedman, D.J. and Raper, J. (2014) Evolution of the primate trypanolytic factor APOL1. Proc Natl Acad Sci U S A. 111 E2130-9

Dixon, E.V., Claridge, J.K., Harvey, D.J., Baruah, K., Yu, X., Vesiljevic, S., Mattick, S., Pritchard, L.K., Krishna, B., Scanlan, C.N., Schnell, J.R., Higgins, M.K., Zitzmann, N. and Crispin M. (2014) Fragments of bacterial endoglycosidase S and immunoglobulin G reveal subdomains of each that contribute to deglycosylation. J Biol Chem. 289 13876-89

Higgins, M.K. and Carrington, M. (2014) Sequence variation and structural conservation allows development of novel function and immune evasion in parasite surface protein families. Protein Sci. 23 354-365.

Adams, Y., Khunrae, P., Higgins, M.K., Ghumra, A., Rowe, J.A. (2014) Plasmodium falciparumrosetting infected erythrocytes bind to human brain microvascular endothelial cells in vitro demonstrating a dual adhesion phenotype mediated by distinct PfEMP1 domains. Infect Immun. 82 949-959.

 

 

2013

Smith, J.D. Rowe, J.A., Higgins, M.K. and Lavstsen, T. (2013) Malaria's deadly grip: cytoadhesion of Plasmodium falciparum-infected erythrocytes. Cell Microbiol. 15 1976-83.

Yu, X. Baruah, K., Harvey, D.J., Vasiljevic, S., Alonzi, D.S., Song, B.D., Higgins, M.K., Bowden, T.A., Scanlan, C.N. and Crispin, M. (2013) Engineering hydrophobic protein-carbohydrate interactions to fine-tune monoclonal antibodies. J Am Chem Soc. 135 9723-32.

Turner, L., Lavstsen, T., Berger, S.S., Wang, C.W., Petersen, J.E.V., Avril, M., Brazier, A.J., Freeth, J., Jespersen, J.S., Nielsen, M.A., Magistrado, P., Lusingu, J., Smith, J.D., Higgins, M.K. and Theander, T.G. (2013) Severe malaria is associated with parasite binding to endothelial protein C receptor. Nature 498 502-5.

Brown, A., Turner, L., Christoffersen, S., Andrews, K.A., Szestak, T., Zhao, Y., Larsen, S., Craig, A.G. and Higgins, M.K. (2013) Molecular architecture of a complex between a PfEMP1 adhesion protein from the malaria parasite and ICAM-1. J. Biol. Chem. 288 5992-6003

Higgins, M.K., Tkachenko, O., Brown, A., Reed, J., Raper, J. and Carrington, M. (2013) Structure of the trypanosome haptoglobin-hemoglobin receptor and implications for nutrient uptake and innate immunity. Proc. Natl. Acad. Sci USA 110 1905-1910

Bengtsson, A., Joergensen, L., Rask, T.S., Olsen, R.W., Andersen, M.A., Turner, L., Theander, T.G., Hviid, L., Higgins, M.K., Craig, A., Brown, A. and Jensen, A.T. (2013) A Novel Domain Cassette Identifies Plasmodium falciparum PfEMP1 Proteins Binding ICAM-1 and Is a Target of Cross-Reactive, Adhesion-Inhibitory Antibodies. J Immunol. 190 240-9

 

 

Before 2012

Glaser, S., van Dooren, G.G., Agrawal, S., Brooks, C.F., McFadden, G.I., Striepen, B. and Higgins, M.K. (2012) Tic22 is an essential chaperone required for protein import into the apicoplast. J. Biol. Chem. 287 39505-12

Glaser, S. and Higgins, M.K. (2012) Overproduction, purification and crystallization of PfTic22, a component of the import apparatus from the apicoplast of Plasmodium falciparum. Acta Cryst. F68 351-354

Ghumra, A., Khunrae, P., Ataide, R., Raza, A., Rogerson, S.J., Higgins, M.K. and Rowe, J.A. (2011) Immunisation with recombinant PfEMP1 domains elicits functional rosette-inhibiting and phagocytosis-inducing antibodies to Plasmodium falciparum. PLoS ONE 6 e16414

Barfod, L., Dobrilovic, T., Magistrado, P., Khunrae, P., Viwami, F., Bruun, J., Dahlbäck, M., Bernasconi, N.L., Fried, M., John, D., Duffy, P.E., Salanti, A., Lanzavecchia, A., Lim, C.T., Ndam, N.T., Higgins, M.K. and Hviid L. (2010) CSA-adhering Plasmodium falciparum-infected erythrocytes express functionally important antibody epitopes shared by multiple variants. J. Immunol 185 7553-7561

Brown, A. and Higgins, M.K. (2010) Carbohydrate binding molecules in malaria pathology. Curr. Op. Struct. Biol. 20 560-566

Khunrae, P. and Higgins, M.K. (2010) Structural insights into chondroitin sulphate binding in pregnancy-associated malaria. Biochem. Soc. Trans. 38 1337-1341

Khunrae, P., Dahlbäck, M., Nielsen, M.A., Andersen, G., Ditlev, S.B., Resende, M., Pinto, V.V., Theander, T.G., Higgins, M.K. and Salanti, A. (2010) Full-length recombinant Plasmodium falciparum VAR2CSA binds specifically to CSPG and induces potent parasite adhesion blocking antibodies. J. Mol. Biol. 397 826-34

Khunrae, P., Philip, J.M.D., Bull, D.R. and Higgins, M.K. (2009) Structural comparison of two CSPG-binding DBL domains from the VAR2CSA protein important in malaria during pregnancy. J. Mol. Biol. 393 202-213

Higgins, M.K. (2008) The structure of a chondroitin sulphate-binding domain important in placental malaria. J. Biol. Chem. 283 21842-21846

Higgins, M.K. (2008) Overproduction, purification and crystallization of a chondroitin-sulphate A-binding DBL domain from a Plasmodium falciparum var2csa-encoded PfEMP1 protein. Acta Cryst. F64 221-223

Higgins, M.K., Oprian, D.D. and Schertler, G.F.X. (2006) Recoverin binds exclusively to an amphipathic peptide at the N-terminus of rhodopsin kinase, inhibiting rhodopsin phosphorylation without affecting catalytic activity of the kinase. J Biol Chem. 281 19426-32

Higgins, M.K. and McMahon, H.T. (2005) In vitro reconstitution of discrete stages of dynamin-dependent endocytosis. Methods. Enzymol. 404 597-611

Eswaran, J., Koronakis, E., Higgins, M.K., Hughes, C. and Koronakis, V. (2004) Three's company: component structures bring a closer view of tripartite drug efflux pumps. Curr. Op. Struct. Biol. 14 741-747

Higgins, M.K., Eswaran, J., Edwards, P.C., Schertler, G.F.X, Hughes, C. and Koronakis, V. (2004) Structure of the ligand-blocked periplasmic entrance of the bacterial multidrug efflux protein TolC. J. Mol. Biol. 342 697-702

Higgins, M.K., Bokma, E., Koronakis, E., Hughes, C. and Koronakis, V. (2004) Structure of the periplasmic component of a bacterial antibiotic efflux pump. Proc. Natl. Acad. Sci. USA 101 9994-9999

Claret, L., Calder, S.R., Higgins, M.K. and Hughes, C. (2003) Oligomerisation and activation of the FliI ATPase central to bacterial flagellum assembly. Mol. Microbiol. 48 1349-1355

Higgins, M.K., Demir, M. and Tate, C.G. (2003) Calnexin co-expression and the use of weaker promoters increase the expression of correctly assembled Shaker potassium channel in insect cells. B.B.A. 1610 124-132

Higgins, M.K., Weitz, D., Warne, T., Schertler, G.F.X. and Kaupp, U.B. (2002) Molecular architecture of a retinal cGMP-gated channel: the arrangement of the cytoplasmic domains. E.M.B.O. Journal 21 2087-94

Higgins, M.K. and McMahon, H.T. (2002) Snap-shops of clathrin-mediated endocytosis. Trends. Biochem. Sci. 27 257-63

Sawle, A., Higgins, M.K., Olivant, M.P. and Higgins, J.P. (2002) A rapid single-step centrifugation method for determination of HDL, LDL and VLDL cholesterol and TG, and identification of predominant LDL subclasses. J. Lipid Research 43 335-43

Ford, M.G.J., Pearse, B.M.F., Higgins, M.K., Vallis, Y., Owen, D., Gibson, A., Hopkins, C.R., Evans, P.R. and McMahon, H.T. (2001) Simultaneous binding of PtdIns(4,5)P2 and clathrin by AP180 in the nucleation of clathrin lattices on membranes. Science 291 1051-5